Subject(s)
Adenocarcinoma/diagnosis , Adenocarcinoma/pathology , Adenocarcinoma/physiopathology , Adenocarcinoma/surgery , Biopsy , Diagnosis, Differential , Humans , Laryngeal Mucosa/pathology , Magnetic Resonance Imaging , Male , Middle Aged , Neoplasm Grading , Otorhinolaryngologic Surgical Procedures , Palatal Neoplasms/diagnosis , Palatal Neoplasms/pathology , Palatal Neoplasms/physiopathology , Palatal Neoplasms/surgery , Palatal Obturators , Recovery of Function , Tomography, X-Ray ComputedABSTRACT
PURPOSE: To evaluate swine vocal fold healing in a period of 30 days after topical mitomycin-C application. METHODS: Twelve swine underwent exeresis of mucosal flaps from the free edge of the anterior third of both vocal folds with a cold instrument (laryngeal scissors). The animals were divided into two groups: EG (Experimental Group), consisting of 6 animals undergoing topical MMC application (4 mg/dL) on the operated area for 4 minutes; CG (Control Group), 6 animals undergoing topical saline solution application on the operated area for 4 minutes. After 30 days, the animals were sacrificed and the larynges were collected and examined for the presence of synechiae as well as a histological immunohistochemical assessment of immature and mature collagen deposition, number of blood vessels and myofibroblasts. RESULTS: Mature collagen deposition in the EG was 452.12 μm² and 1332.31μm² in the CG; immature collagen deposition was 1511.73μm² in the EG and 1020.61μm² in the CG. The number of myofibroblasts was 1.556 in the EG and 3.583 in the CG. The number of blood vessels was 2.565 in EG and 6.917 in the CG. There were no synechiae in the two studied groups. CONCLUSIONS: There was an increase in immature collagen deposition in the experimental group when compared with the control group. There was a decrease in mature collagen deposition in the experimental group when compared with the control group. There was a decrease in the number of myofibroblasts in the experimental group when compared with the control group. A decrease in blood vessels was observed in the experimental group when compared with the control group. There was no synechia formation in either studied group.
OBJETIVO: Avaliar a cicatrização de pregas vocais de suínos utilizando mitomicina-C tópica, em 30 dias. MÉTODOS: Doze suínos foram submetidos à exérese de mucosa do bordo livre do terço anterior de ambas as pregas vocais com instrumental frio (tesoura curva) e divididos em dois grupos: Grupo E (GE), composto por seis animais com utilização tópica de mitomicina-C(4 mg/dL) na área cruenta por quatro minutos e Grupo Controle (GC), composto por seis animais com utilização tópica de soro fisiológico na área cruenta por quatro minutos. Após 30 dias os animais foram sacrificados e coletadas amostras das pregas vocais para avaliação de sinéquias e análise histológica com quantificação da deposição de colágeno maduro e imaturo, quantificação do número de vasos sanguíneos e miofibroblastos, por método imunoistoquímico. RESULTADOS: A deposição do colágeno maduro no GE foi de 452,12 μm² e 1332,31μm² no GC. A do colágeno imaturo foi de 1511,73μm² no GE e de 1020,61μm² no GC. O número de miofibroblastos foi de 1,556 no GE e de 3,583 no GC. O número de vasos sanguíneos foi de 2,565 no GE e de 6,917 no GC. Não houve formação de sinéquias nos grupo s estudados. CONCLUSÕES: No grupo experimento, quando comparado ao controle, houve significativamente aumento da deposição do colágeno imaturo e diminuição da deposição do colágeno maduro, do número de miofibroblastos e de vasos sanguíneos. Não houve formação de sinéquias em ambos os grupos.
Subject(s)
Animals , Female , Male , Laryngeal Mucosa/drug effects , Mitomycin/pharmacology , Vocal Cords/blood supply , Vocal Cords/surgery , Wound Healing/drug effects , Collagen/biosynthesis , Collagen/classification , Fibrin/biosynthesis , Laryngeal Mucosa/pathology , Models, Animal , Neovascularization, Physiologic , Random Allocation , Swine , Tissue AdhesionsABSTRACT
BACKGROUND AND AIMS: Recent studies have reported high prevalence rates of short segments of specialized columnar epithelium (SCE) in the distal esophagus. The association of SCE with gastroesophageal reflux disease is not well established. We studied the prevalence and associations of short segments of SCE in the distal esophagus amongst Indians. METHODS: 271 patients (mean age 36 [14] y; 160 men) undergoing diagnostic upper gastrointestinal endoscopy were interviewed regarding symptoms of gastroesophageal reflux, and history of medications, smoking or chewing tobacco and alcohol ingestion. At endoscopy, presence and grade of esophagitis and hiatus hernia were recorded. One biopsy each was taken from the squamocolumnar junction and 2 cm proximal to it. Biopsies were stained with hematoxylin/eosin and alcian blue/periodic acid-Schiff. The pathologist was blinded to the clinical and endoscopic data. RESULTS: Short segments of SCE in the distal esophagus were present in 16/271 (6%; CI 5.03-6.97) patients. Increasing age (p<0.01), and endoscopic (p<0.01) and histologic (p<0.001) esophagitis were associated with its presence, whereas symptoms of gastroesophageal reflux, smoking, tobacco chewing, use of alcohol or non-steroidal anti-inflammatory drugs, and hiatus hernia were not. One patient with SCE had dysplasia. CONCLUSION: Prevalence of short segments of SCE in the distal esophagus amongst Indians is low and is usually associated with inflammation in the esophagus.
Subject(s)
Adult , Aged , Alcian Blue , Barrett Esophagus/etiology , Confidence Intervals , Endoscopy , Esophagus/chemistry , Female , Gastroesophageal Reflux/complications , Humans , Laryngeal Mucosa/pathology , Male , Middle Aged , Periodic Acid-Schiff Reaction/methods , PrevalenceABSTRACT
Os autores submeteram 30 cäes à intubaçäp endotraqueal com cânulo de alta complacência, durante período de quatro horas, com o objetivo de detetar lesöes precoces laringotraqueais secundárias à intubaçäo. Após este período, realizaram-se biópsias em cordas vocais, aritenóides, cricóide e anéis traqueais. As alteraçöes histológicas mais frequentemente observadas foram: infiltrado de células polimorfonucleares, tanto no epitéleio como no córion, congestäo vascular e necrose de células epiteliais. Os anéis traqueais, que se encontravam em íntimo contacto com o balonete da cânula, foram os locais mais comprometidos. Os autores descrevem os principais fatores lesivos ao epitélio respiratório e preconizam a utilizaçäo de cânulas com balonetes de alta complacência, além do seguimento endoscópico dos pacientes após extubaçäo
Subject(s)
Animals , Male , Female , Dogs , Intubation, Intratracheal/adverse effects , Larynx/pathology , Trachea/pathology , Laryngeal Mucosa/pathology , Time FactorsABSTRACT
Tabagismo e mucosa laringeana, säo discutidas, sob os aspectos patogênicos. Os pólipos da laringe säo descritos como uma reaçäo mucosa ao fumo